Summary
Type: SPE
Application #: 301140
Catalog#: 730013 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Column conditioning:
2 column volumes methanol, then 2 column volumes dist. water pH 7
Sample application:
Slowly force or aspirate the sample through the column
Column washing:
2 column volumes water (if necessary, adjust pH with hydrochloric acid or
ammonia as indicated above); dry column under vacuum for 5 min
Elution:
Aspirate 750 μl eluent into the column packing, after 1 min elute and flush
with another portion of 750 μl eluent
Eluents: acetone for cannabinoids from blood
acetone / chloroform (1 : 1, v/v) for barbiturates, active components in
general, bases and amphetamines
Authors: Macherey-Nagel
Source: LC Department